Specialized Tools for Electrophysiology and Cell Biology Research

(RC-27) Large Rectangular Open Bath Chamber

Product Summary

A large bath chamber suitable for studies requiring smooth solution flow with a large viewing area.

  • Accommodates large tissue sections or brain slices
  • Large rectangular bath
  • Large optical imaging area
  • Can be used with either upright or inverted microscopes
  • Slice anchors available

The RC-27 is a large bath chamber ideal for large tissue or brain slice studies. The large imaging area increases the likelihood of viewing desired features in the angled sides of the chamber facilitate electrode placement.

The chamber floor consists of a 24 x 50 mm rectangular coverslip and the bath area is 12 x 24mm. these dimensions are useful for organotypic slice culture studies. An adjustable spreader plate at the bath input facilitates uniform solution flow. The working volume of the RC-27 is 413 ul per mm of solution height. The chamber is equipped with a stainless steel aspiration tube together with a clamp.

Designed for brain or tissue slice studies, several optional slice anchors are offered with Lycra thread spacings of 1.0, 1.5, or 2.0 mm. An anchor kit is also available containing slice anchors (1 each) in each available thread spacing.

The RC-27 requires a platform (PM-6PH-6 or P-6) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

 

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, 50/pkg) and 10 ft of PE-160 tubing. Available slice anchors sold separately.

Material Polycarbonate
Bath dimensions
(L x W x H)
38 x 12.5 x 4 mm
Volume, by depth 413 μl/mm
Bottom coverglass 24 x 50 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

 

 

Order No. Model No. Product Description USA Price Order
64-0708 CS-22/50 Rectangular cover glass, #1 thickness, 22 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Effects of Oleic Acid on Distinct Populations of Neurons in the Hypothalamic Arcuate Nucleus Are Dependent on Extracellular Glucose Levels,
    R. Wang1, C. Cruciani-Guglielmacci2, S. Migrenne2, C. Magnan2, V. E. Cotero1 and V. H. Routh1,
    J Neurophysiol 95: 1491-1498, 2006. First published November 23, 2005
  2. Reactive oxygen species formation in the transition to hypoxia in skeletal muscle,
    Li Zuo and Thomas L. Clanton,
    Am J Physiol Cell Physiol 289: C207-C216, 2005

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
Chamber
64-0238 RC-27 Very large bath recording chamber Login Login
Platforms
64-1527 PM-6 PM-6 Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-0289 PH-6 PH-6 Platform for Series 20 chambers, heater Login Login
64-0282 P-6 P-6 Platform for Series 20 chambers, non-heater Login Login
Slice Anchors (Harps)
64-0267 SHD-27KIT Slice anchor kit for RC-27, stainless steel Login Login
64-0194 SHD-27H/10 Slice anchor for RC-27, 1.0 mm thread spacing, stainless steel Login Login
64-0193 SHD-27H/15 Slice anchor for RC-27, 1.5 mm thread spacing, stainless steel Login Login
64-0192 SHD-27H/2 Slice anchor for RC-27, 2.0 mm thread spacing, stainless steel Login Login
Replacement Consumables
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Temperature Control
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
Accessories
64-1940 DWV Dedicated Workstation Vacuum Login Login
64-1920 SA-20PS1 Adapter Ring for Pecon Heating Insert P Login Login
Replacement Parts
64-1451 CPS-27 Perfusion spreader plate for RC-27 chamber Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login

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