Specialized Tools for Electrophysiology and Cell Biology Research

(RC-28) Excised Patch Chamber with Two Slotted Open Baths

Product Summary

The dual slotted open bath construction of the excise patch chamber permits exchange of solution surrounding a membrane patch without contamination of the associated cell population.

  • Designed for excised patch studies
  • Open chamber design
  • Dual slotted baths in two sizes
  • Baths separated by a controllable dam

The RC-28 is a special chamber containing two bath compartments separated by an adjustable dam. The dam functions both as a gate and barrier between the baths. The composition and flow rate of each bath can be independently controlled, and each bath has a separate stainless steel suction tube for perfusion.

The dual slotted, open bath design allows for the exchange of solution surrounding an isolated membrane patch without contaminating or modifying the rest of the population. Raising the solution level in the dam area joins the two baths, while lowering the solution level isolates the two baths. After patching onto a cell in the large bath compartment, the dam is flooded, enabling secure transfer of the electrode attached cell to the smaller compartment. The dam is then drained isolating the two baths.

For the larger chamber, the bath area is 21 x 9.5 mm and the working volume is 243 ul per 1.0 mm of solution height. For the smaller chamber, the bath area is 28 x 3.8 mm and the volume is 111 ul.

A platform (PM-6PH-6 or P-6) and Series 20 stage adapter are required to complete the assembly for mounting onto a microscope. The chamber bottom is formed by a 24 x 50 mm coverslip.

 

The RC-28 is supplied with a pack of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, 50/pkg) and 10 ft of PE-160 polyethylene tubing.

Material Polycarbonate
Dimensions, small bath d
(L x W x H)
20.0 x 3.8 x 4.5 mm
Dimensions, large bath d
(L x W x H)
28.0 x 9.5 x 4.5 mm
Volume, small bath, by depth 111 µl
Volume, large bath, by depth 243 µl
Specimen area, small bath (L x W) 28 x 3.8 mm
Specimen area, large bath (L x W) 21 x 9.5 mm
Bottom coverglass 24 x 50 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

 

 

RC-28 line drawing

 

 

Order No. Model No. Product Description USA Price Order
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Barium, TEA and sodium sensitive potassium channels are present in the human placental syncytiotrophoblast apical membrane
    Díaz P, Vallejos C, Guerrero I, Riquelme G.
    Placenta. 2008 Oct;29(10):883-91
  2. The maxi-chloride channel in human syncytiotrophoblast: a pathway for taurine efflux in placental volume regulation
    Vallejos C, Riquelme G
    Placenta. 2007 Nov-Dec;28(11-12):1182-91
  3. Correlative Studies of Gating in Cx46 and Cx50 Hemichannels and Gap Junction Channels
    Miduturu Srinivas, Jack Kronengold, Feliksas F. Bukauskas,y Thaddeus A. Bargiello, and Vytas K. Verselisy
    Biophysical Journal Volume 88 March 2005 1725–1739

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
Chamber
64-0243 RC-28 Excised patch recording chamber Login Login
Platforms
64-1527 PM-6 PM-6 Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-0289 PH-6 PH-6 Platform for Series 20 chambers, heater Login Login
64-0282 P-6 P-6 Platform for Series 20 chambers, non-heater Login Login
Replacement Consumables
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Temperature Control
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories
64-1940 DWV Dedicated Workstation Vacuum Login Login
Replacement Parts
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

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