Specialized Tools for Electrophysiology and Cell Biology Research

(RC-43C) Closed Bath Chamber

Product Summary

Designed for multiple successive experiments requiring the fast exchange of #1 or #1.5 round coverslips.

 

  • Dual O-ring seal system enables quick coverslip exchange that's ideal for rapid screening assays
  • Closed bath design promotes both smooth continuous solution exchange and a stable imaging focus
  • Anodized aluminum base insures good heat transfer

Warner Instruments is pleased to introduce a new family of chambers designed to meet the need for rapid screening of cells grown on coverslips. The innovative design uses O-rings to seal the coverslip to the polycarbonate chamber. Therefore little or no silicone grease is needed and mechanical fasteners are not required for coverslip mounting. The chamber design consists of two parts; a polycarbonate chamber and a 40 mm diameter aluminum base.

The RC-43C closed bath chamber is supplied with one package each of #1.5 coverslips, 25 and 18 mm round, respectively, and spare O-rings. The distance between top and bottom coverslips is 1 mm. Additionally this chamber is also supplied with 10 feet of PE-160 and PE-90 polyethylene tubing.

The RC-43C closed bath design promotes an even solution exchange and eliminates changes in imaging focus due to alterations in bath height. Studies involving physiological bicarbonate buffers can be performed with no gas loss at the chamber interface.

Fluid is delivered to a 20 gauge stainless steel perfusion inlet port and removed from the chamber by means of a similar outlet port. The top coverslip is held in place with a retainer, a thin coating of silicone grease forms the seal between the chamber and the coverglass.

Series 40 chambers are designed to be compatible with Warner's QE-1 Quick Exchange Platform and DH-35iL and DH-40iL culture dish incubators which are compatible with Warner Series 20 microscope stage adapters.

A temperature controller TC-344C or TC-324C that connects to the heated platform QE-1 or the microincubators DH-35iL and DH-40iLcan be added to set and maintain a certain temperature.

Perfusion control can be achieved by adding a VCS Perfusion System, the DVW vacuum system takes care of the waste.

The RC-43C Chamber is supplied with one package each of #1.5 coverslips (25 and 18 mm round, respectively) and spare O-rings. Additionally, the RC-43C is supplied with 10 feet of PE-160 and PE-90 polyethylene tubing.

Model Bottom
Coverslip
Top
Coverslip
Aperture Total
Volume
RC-43C 25 mm 18 mm 15.8 mm 213 ¼L

 

 

Order No. Model No. Product Description USA Price Order
64-0349 DH-35iL Culture dish incubation system Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-0102 SH-27B In-line solution heater Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-1492 OR-2 Replacement Retaining Ring o-ring for all RC-40 Series, 5 pack, black Login Login
64-0375 QE-1 Quick change platform, heated base, for RC-40 and QR-40 series chambers Login Login
64-1495 OR-5 Replacement o-ring for QR/RC-43C, 5 pack, red Login Login

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
64-0371 RC-43C Quick change chamber for 25 mm coverslips, closed bath Login Login
Accessories and Replacement Parts
64-1495 OR-5 Replacement o-ring for QR/RC-43C, 5 pack, red Login Login
64-1492 OR-2 Replacement Retaining Ring o-ring for all RC-40 Series, 5 pack, black Login Login
Temperature Control
64-0375 QE-1 Quick change platform, heated base, for RC-40 and QR-40 series chambers Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0102 SH-27B In-line solution heater Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-1921 SA-40PS1 Adapter Ring for Pecon Heating Insert P Login Login
64-0349 DH-35iL Culture dish incubation system Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login
Glass Coverslips
64-0715 CS-25R15 Round cover glass, #1.5 thickness, 25 mm, 100 pack Login Login
64-0714 CS-18R15 Round cover glass, #1.5 thickness, 18 mm, 100 pack Login Login
Perfusion and Waste Management
64-1940 DWV Dedicated Workstation Vacuum Login Login
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login

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