Specialized Tools for Electrophysiology and Cell Biology Research

PE 30 Series for Microperfusion Applications

Product Summary

Electrode holders designed to allow perfusion inside the micropipette while applying pressure or suction in the presence of an electrical connection

  • Machined in polycarbonate for low noise recording in patch clamp
  • Accepts 1.0, 1.2, 1.5, 1.7, or 2.0 mm OD capillaries
  • Gold-plated connector pins with PTFE or threaded polycarbonate insulators
  • 30° body style with Ag wire electrode
  • Includes sealed perfusion port and suction port

Electrode holders specially designed for microperfusion applications!

Patch Clamp Compatibility
Electrical Connector Compatibility
1 mm pin Current Warner and pre-1995 Axon patch clamps
1 mm pin with threaded collar All Axon amplifiers made after February 1995
BNC compatible List, Heka, NPI, and Dagan patch clamps

PE Series Holders for perfusion applications

Perfusion at the electrode tip is achieved via an additional port positioned in-line with the glass bore that allows for the insertion of microbore tubing into the pipette. This port includes a threaded cap and seal for up to 1 mm diameter tubing.

Electrical Connector Glass OD (mm) Model Order No. Quick Add
to Order
Image
1 mm pin 1.0 PE30W-A10P 64-1761 Login 641763_PESWA15P
Threaded collar with 1 mm pin 1.0 PE30W-T10P 64-1771 Login 641773_PESWT15P
1.2 PE30W-T12P 64-1772 Login
1.5 PE30W-T15P 64-1773 Login
1.7 PE30W-T17P 64-1774 Login
2.0 PE30W-T20P 64-1775 Login
BNC 1.0 PE30W-B10P 64-1766 Login 641768_PESWB15P
1.2 PE30W-B12P 64-1767 Login
1.5 PE30W-B15P 64-1768 Login
1.7 PE30W-B17P 64-1769 Login
2.0 PE30W-B20P 64-1770 Login
Body Material Polycarbonate
Body Style 30°
Electrical Connectors 1 mm pin with PTFE insulator
BNC compatible PTFE with 1.2 mm pin
Threaded polycarbonate collar with 1 mm pin
Electrode Coupling Ag wire
Pressure Port Polycarbonate, 2 mm OD
Perfusion Port Accepts tubing up to 1 mm OD
Glass Capillary Sizes 1.0, 1.2, 1.5, 1.7 and 2.0 mm

References

References

  1. Nehr, E. and Eckert, R.: Fast patch-pipette internal perfusion with minimum solution flow. Grinnel, A.D., Armstrong, D. and Jackson, M.B.: Calcium and Ion Channel Modulation, Plenum Press, N.Y.
  2. Cull-Candy, S.G., Miledi, R. and Parker, I.: Single glutamate-activated channels recorded from locust muscle fibers with perfused patch clamp electrodes. J. Physiology 32-, 195-210.
  3. Pusch, M. and Nehr, E. (1987) Kinetics of loading small cells with various compounds by use of patch pipettes. Pflugers Archives (Spring Meeting of the Physiol. Ges.).
  4. Tang, J.M., Wang, J. and Eisenberg, R.S. (1992) Perfusing patch pipettes. Methods in Enzymol. 207, 176-181.

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
1 mm pin with PTFE insulator
64-1761 PE30W-A10P PE Series electrode holder, port, 30 style, fits 1.0 mm capillary, 1 mm Pin Login Login
Threaded collar with 1 mm pin
64-1771 PE30W-T10P PE Series electrode holder, port, 30 style, fits 1.0 mm capillary, 1 mm pin threaded collar Login Login
64-1772 PE30W-T12P PE Series electrode holder, port, 30 style, fits 1.2 mm capillary, 1 mm pin threaded collar Login Login
64-1773 PE30W-T15P PE Series electrode holder, port, 30 style, fits 1.5 mm capillary, 1 mm pin threaded collar Login Login
64-1774 PE30W-T17P PE Series electrode holder, port, 30 style, fits 1.7 mm capillary, 1 mm pin threaded collar Login Login
64-1775 PE30W-T20P PE Series electrode holder, port, 30 style, fits 2.0 mm capillary, 1 mm pin threaded collar Login Login
BNC compatible PTFE with 1.2 mm pin
64-1766 PE30W-B10P PE Series electrode holder, port, 30 style, fits 1.0 mm capillary, BNC Login Login
64-1767 PE30W-B12P PE Series electrode holder, port, 30 style, fits 1.2 mm capillary, BNC Login Login
64-1768 PE30W-B15P PE Series electrode holder, port, 30 style, fits 1.5 mm capillary, BNC Login Login
64-1769 PE30W-B17P PE Series electrode holder, port, 30 style, fits 1.7 mm capillary, BNC Login Login
64-1770 PE30W-B20P PE Series electrode holder, port, 30 style, fits 2.0 mm capillary, BNC Login Login
Accessories
64-0167 TY-50 Tygon tubing, 1/16" x 1/8" x 50 ft (ID x OD x length) Login Login

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