Specialized Tools for Electrophysiology and Cell Biology Research

(RC-27NE2) Narrow Bath Chamber with Field Stimulation

Product Summary

This chamber has a narrower bath and smaller volume than the RC-27. It also has embedded electrodes to support studies using field stimulation.

  • Designed for field stimulation studies
  • Rectangular, open-bath chamber
  • Narrow bath
  • Accommodates tissue or brain slice specimens
  • Applications include patch clamp and physiological measurements on cultured cells

The RC-27NE2 recording chamber is a modified version of the RC-27 with a narrower bath and smaller volume designed for applications requiring electric field stimulation. Laminar solution flow is more stable in this chamber than in the wider RC-27 chamber.

The working specimen area is 28 x 8 mm and there are inclined surfaces on both sides (from 2.0 mm to 3.1 mm height). The low profile design and inclined surfaces permits good electrode access to the specimen area.

The chamber is supplied with a pair of platinum wire electrodes installed along the parallel long sides of the bath. Electrode leads are approximately 16 cm long and terminate with 1 mm diameter pins. The RC-27NE2 is compatible with all available field stimulators.

Narrow Bath Chamber with Field Stimulation (RC-27NE2)

The RC-27NE2 chamber requires a platform (PM-6PH-6 or P-6) and stage adapter to complete the assembly for mounting onto a microscope. The chamber body is manufactured from polycarbonate and the chamber floor is formed from a 24 x 50 mm coverslip.

Narrow Bath Chamber with Field Stimulation (RC-27NE2)

Narrow Bath Chamber with Field Stimulation (RC-27NE2)
Narrow Bath Chamber with Field Stimulation (RC-27NE2)

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, pkg. of 50) and 10 ft of PE-160 polyethylene tubing.

Material Polycarbonate
Bath dimensions
(L x W x H)
37 x 8 x 2 mm
Outer dimensions
(L x W x H)
50 x 25 x 4 mm
(with 8 mm boss to hold suction tube)
Volume, by depth 305 μl/mm
Electrodes Platinum wire
16 mm from chamber to 1 mm pins
23 mm length in bath
Bottom coverglass 24 x 50 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

 

 

Order No. Model No. Product Description USA Price Order
64-1942 SE-27NE-REP Replacement Stimulation Electrodes for RC-27NE2 Login Login
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
89-0706 STG4002-1.6mA Stimulus generator, 2-channel, 4000 Series, ±1.6 mA Login Login
89-0707 STG4002-160µA Stimulus generator, 2-channel, 4000 Series, ±160 µA Login Login
89-0708 STG4002-16mA Stimulus generator, 2-channel, 4000 Series, ±16 mA Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Multiple pathological events in exercised dystrophic mdx mice are targeted by pentoxifylline: outcome of a large array of in vivo and ex vivo tests
    Burdi R, Rolland JF, Fraysse B, Litvinova K, Cozzoli A, Giannuzzi V, Liantonio A, Camerino GM, Sblendorio V, Capogrosso RF, Palmieri B, Andreetta F, Confalonieri P, De Benedictis L, Montagnani M, De Luca A
    J Appl Physiol. 2009 Apr;106(4):1311-24
  2. Gentamicin treatment in exercised mdx mice: Identification of dystrophin-sensitive pathways and evaluation of efficacy in work-loaded dystrophic muscle
    De Luca A, Nico B, Rolland JF, Cozzoli A, Burdi R, Mangieri D, Giannuzzi V, Liantonio A, Cippone V, De Bellis M, Nicchia GP, Camerino GM, Frigeri A, Svelto M, Camerino DC.
    Neurobiol Dis. 2008 Nov;32(2):243-53
  3. Fluvastatin and Atorvastatin Affect Calcium Homeostasis of Rat Skeletal Muscle Fibers in Vivo and in Vitro by Impairing the Sarcoplasmic Reticulum/Mitochondria Ca2+-Release System
    Liantonio A, Giannuzzi V, Cippone V, Camerino GM, Pierno S, Camerino DC.
    J Pharmacol Exp Ther. 2007 May;321(2):626-34
  4. Niflumic acid inhibits chloride conductance of rat skeletal muscle by directly inhibiting the CLC-1 channel and by increasing intracellular calcium
    Liantonio A, Giannuzzi V, Picollo A, Babini E, Pusch M, Conte Camerino D
    Br J Pharmacol. 2007 Jan;150(2):235-47
  5. Role of tumour necrosis factor [alpha], but not of cyclo-oxygenase-2-derived eicosanoids, on functional and morphological indices of dystrophic progression in mdx mice: a pharmacological approach
    Pierno S, Nico B, Burdi R, Liantonio A, Didonna MP, Cippone V, Fraysse B, Rolland JF, Mangieri D, Andreetta F, Ferro P, Camerino C, Zallone A, Confalonieri P, De Luca A
    Neuropathol Appl Neurobiol. 2007 Jun;33(3):344-59

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
Chambers
64-0240R2 RC-27NE2 Narrow Bath Recording Chamber w/ Field Stimulation Login Login
Platforms
64-1527 PM-6 PM-6 Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-0289 PH-6 PH-6 Platform for Series 20 chambers, heater Login Login
64-0282 P-6 P-6 Platform for Series 20 chambers, non-heater Login Login
Stimulators - Bipolar
89-0707 STG4002-160µA Stimulus generator, 2-channel, 4000 Series, ±160 µA Login Login
89-0706 STG4002-1.6mA Stimulus generator, 2-channel, 4000 Series, ±1.6 mA Login Login
89-0708 STG4002-16mA Stimulus generator, 2-channel, 4000 Series, ±16 mA Login Login
64-1424 SIU-102 Bipolar stimulator, high current, non-isolated Login Login
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
65-0364 DS4 Bipolar stimulator, constant current, isolated Login Login
Stimulators - Monopolar
89-0707 STG4002-160µA Stimulus generator, 2-channel, 4000 Series, ±160 µA Login Login
89-0706 STG4002-1.6mA Stimulus generator, 2-channel, 4000 Series, ±1.6 mA Login Login
89-0708 STG4002-16mA Stimulus generator, 2-channel, 4000 Series, ±16 mA Login Login
64-1933 SIU-202 Monopolar stimulator, constant current, isolated Login Login
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
65-0550 DS3 Monopolar stimulator, constant current, isolated Login Login
65-0329 DS2A-MKII Monopolar stimulator, constant voltage, isolated Login Login
Replacement Consumables
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Temperature Control
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Replacement Parts
64-1942 SE-27NE-REP Replacement Stimulation Electrodes for RC-27NE2 Login Login
64-1452 CPS-27N Perfusion spreader plate for RC-27N and RC-27NE2 Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login

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