Specialized Tools for Electrophysiology and Cell Biology Research

(MS-200, MS-200A) Mitocell Respirometry System

Product Summary

The Mitocell Respirometry System is a complete package designed to accommodate automatic oxidative phosphorylation measurements in both isolated mitochondria and intact cells.

The MS-200 and MS-200A Mitocell Respirometry System is the system of choice if you need to make oxidative phosphorylation measurements in either isolated mitochondria or intact cells.

The two systems offered are identical with the exception of the included Mitocell. The MS-200 incorporates the low volume MT-200 Mitocell, and the MS-200A incorporates the larger volume MT-200A Mitocell. The MT-200 has working volumes of 50 and 100 ul, while the MT-200A has working volumes of 300, 500, and 1000 ul.

The special software powers a dedicated platform for mitochondrial research and will calculate State 3 and State 4 respiration rates, P/O ratios and RCR. The program also records and automatically calculates general respiration rates and specific oxygen uptake rates.

Applications include:
  • Mitochondrial dysfunction characterization
  • Cytotoxicology research with cultured cells
  • Respiration of permeabilised liver tissue and muscle fibers
  • Bactericidal cytotoxicity testing
  • Cell respiration in apoptosis, oxidative stress, and inflammation
  • Oxygen uptake in isolated enzyme systems and sub-mitochondrial particles

The Mitocell MS-200 includes a 782 oxygen meter, a 1302 oxygen electrode, an MT-200 or MT-200A respirometer, and special respirometry software specifically designed for making oxidative phosphorylation measurements. The system also includes an electrode service kit and an easy-to-follow manual.

Note: You have the option to purchase a second Mitocell and 1302 electrode to take advantage of the capacity of the 782 meter and software to record from 2 electrodes simultaneously.

Specifications for MT-200 and MT-200A

  MT-200 MT-200A
Sample chamber 50 µl, 100 µl 300 µl, 500 µl, 1000 µl
Diameter 4 mm 4 mm
Chamber unit Glass Glass
Plunger Polycarbonate Polycarbonate
Bore 1 mm 1 mm
Magnetic stirrer 5 volt DC from plug-top power supply 5 volt DC from plug-top power supply
Shipping Dimensions 12 x 12 x 12 inches 12 x 12 x 12 inches
Shipping Weight 8lbs 8lbs

 

Specifications for 782 Oxygen Sensor

 

Resolution with 1302 electrode 0.1% of full scale
Logging Capacity 2000 readings
Off-line data storage 4 hours at 1/sec reading
PC requirements Windows PC, USB port, with Windows 7 64 bit or earlier; 4 MB of hard disk space and at least 64 MB of RAM
Power 5 VDC; 1 A, w/external plug
Power supply 100-230 VAC, 47-63 Hz
Dimensions 185 x 135 x 105 mm (7.25 x 5.25 x 4.125 in)

 

Specifications for Oxygen Electrode 1302

 

Response time at 37°C:
Polypropylene membranes 18 sec. for 90% change
FEP membranes 6 sec. for 90% change
Oxygen consumption (polypropylene membranes) 0.5 to 3x10-10mg O2/min
Temperature coefficient 2% per °C
Resolution of 928 and 782 meter 0.1% with 1302 electrode*

 

*The display resolution of the 982 and 782 meter is 0.1%. The A/D converter is capable of at least 0.01%, so you can work on an expanded scale. However, the limit is normally set to about 0.1% by the noise level of the 1302 electrode.

 

 

Last Revision 6.28.12

 

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
69-0034 MS-200 Mitocell Precision Respirometry System, 50-100 ul Login Login
69-0035 MS-200A Mitocell Precision Respirometry System, 300-1000 ul Login Login
69-3007 SI-020 Polyproplyene oxygen electrode service kit Login Login
69-3005 SI-021 FEP oxygen electrode service kit Login Login
69-4081 SI-069 Spinbars for MT-200 and MS-200, 6 pack, with magnet Login Login
69-3008 SI-030 Electrolyte solution, 125 ml Login Login
Accessories and Replacement Parts - Oxygen Sensor 1302
69-3014 SI-045 Polyproplyene membraned jackets, box of 6 Login Login
69-3034 SI-023 Polyproplyene membraned jackets, box of 30 Login Login
69-3011 SI-035 Electrode jacket (with hole) for FEP membranes Login Login
69-3010 SI-025 FEP membranes, 15 pack Login Login
69-3036 SI-043 O-rings for FEP membranes, 25 pack Login Login
69-3013 SI-055 O-rings for 1302 electrode body, 25 pack Login Login
69-3035 SI-031 Abrasive membranes, box of 6 Login Login
Accessories and Replacement Parts - MT-200
72-6187 SK-205 Replacement glass and plunger for MT-200 Login Login
69-3038 SI-058 Plunger for MT-200 and MS-200 Login Login
69-3017 SI-066 Modified syringe for MT-200 and MS-200, 1 µl Login Login
69-3040 SI070 Adjustment rod and hex key for MT-200 and MS-200 Login Login
Accessories and Replacement Parts - MT-200A
72-6188 SK-206 Replacement glass and plunger for MT-200A Login Login
69-3037 SI-056 Plunger for MT-200A and MS-200A Login Login
69-3015 SI-067 Modified syringe for MT-200A and MS-200A, 5 µl Login Login
69-3041 SI071 Volume peices and hex key for MT-200A and MS-200A Login Login
69-3039 SI-068 Spinbars for MT-200A, MS-200A, RC-650, and RS-650, 6 pack Login Login
Accessories and Replacement Parts - 782 Oxygen Meter
69-3947 Connecting cable for 782 meter and MS-200/200A cells Login Login
Accessories and Replacement Parts - Thermocirulator
72-4924 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz Login Login
72-4921 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz Login Login
64-2244 WB-KIT TC-120 Thermocirculator Tubing Kit Login Login

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