Specialized Tools for Electrophysiology and Cell Biology Research

(RC-21BDW) Open Diamond Bath Imaging Chamber

Product Summary

An open bath, polycarbonate recording chamber that uses 25 mm round coverslips and incorporates Warner's diamond bath fluidics.

  • Designed for physiological measurements on cell cultured coverslips
  • Identical to the RC-21BRW except for a diamond shaped bath
  • Uses A 25 mm round coverslip as the chamber floor
  • Diamond shaped bath provides laminar solution flow
  • Quiet operation
  • Wicked solution removal from bath
  • Can be used with upright or inverted microscopes

The low profile design of the RC-21BDW allows good electrode access to the chamber bath. Constant solution level and quiet operation are assured with the use of a wick between the bath and suction well. With laminar flow, the sample is minimally distorted during perfusion. Bath volume is 143 µl for each mm solution height.

RC-21BDW chambers require a platform (PM-2PH-2P-2) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

 

Each RC-21BDW chamber is supplied with a package of #1 thickness glass coverslips (CS-25R, 25 mm dia., pkg. of 100) and 10 ft of PE-160 polyethylene tubing.

Material polycarbonate
Bath dimensions
(L x W x H)
17 x 13 x 3.8 mm
Volume, by depth 143 µl/mm
Bottom coverglass 25 mm round
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)


 

Order No. Model No. Product Description USA Price Order
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes
    Shigetomi E, Khakh BS
    J Vis Exp. 2009 Apr 26;(26). pii: 1142. doi: 10.3791/1142
  2. Tissue-specific Regulation of Sodium/Proton Exchanger Isoform 3 Activity in Na+/H+ Exchanger Regulatory Factor 1 (NHERF1) Null Mice
    Murtazina R, Kovbasnjuk O, Zachos NC, Li X, Chen Y, Hubbard A, Hogema BM, Steplock D, Seidler U, Hoque KM, Tse CM, De Jonge HR, Weinman EJ, Donowitz M
    J Biol Chem. 2007 Aug 24;282(34):25141-51. Epub 2007 Jun 19

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
Chamber
64-0362 RC-21BDW Open chamber for 25 mm round coverslip, 100 µl volume Login Login
Platforms
64-1561 PM-2 PM-2 Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-0285 PH-2 PH-2 Platform for Series 20 chambers, heater Login Login
64-0278 P-2 P-2 Platform for Series 20 chambers, non-heater Login Login
Replacement Consumables
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Temperature Control
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories
64-1940 DWV Dedicated Workstation Vacuum Login Login
Replacement Parts
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login

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