Small bath volume facilitates rapid solution exchange
Tissue slice anchor included
The RC-22 and RC-22C feature an open bath and include a slice anchor. The diamond shape geometry of these chamber baths facilitate laminar solution flow which can provide minimal distortion of the sample during perfusion.
Solution aspiration is via a stainless steel suction tube which is secured by a clamp. This insures that the adjustable-height suction tube will not move once set by the user. Each chamber has an agar bridge well for reference electrodes. The working volume of the RC-22 and RC-22C is 115 µl and 138 µl, respectively. Chamber sidewalls have a height of 1.2 mm to facilitate electrode access and placement.
The RC-22 and RC-22C chambers require a platform (PM-1, PH-1 or P-1) and Series 20 stage adapter to complete the assembly for mounting onto a microscope. The bottom-forming coverslip is 22 x 40 mm.
Each chamber is supplied with a slice anchor, a swatch of nylon mesh, a pack of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.
The RC-22 and RC-22C are small volume, open bath recording chambers designed for tissue and brain slice studies.
Designed for tissue slice samples or cell cultured coverslips
Applications include patch clamp, intracellular / extracellular recordings, and imaging