Model RC-26G chamber on a microscope stage with in-line heater and CC-28
cable (a typical set-up)
Model RC-26G chamber shown in a PH-1 heated platform on a SA-NIK stage adapter
The RC-26 and RC-26G chambers are designed to be used with cells cultured onto glass coverslips, or with tissue and brain slice samples. The broad imaging area of these chambers (12 x 25 mm and 15 x 24 mm for the RC-26 and RC-26G, respectively) provides excellent access for both electrodes and water-immersion objectives. The diamond shaped geometry facilitates laminar solution flow throughout the bath area.
The large bath of these chambers allows a coverslip to be inserted into the top opening and removed at the end of the experiment. The RC-26 and RC-26G chambers can accept a 12 or 15 mm diameter coverslip, respectively. The working volumes, for 1 mm solution height, of the RC-26 and RC-26G model chambers are 170 and 234 ul, respectively.
Large tissue samples can also be placed directly into the chamber. Slice anchor hold downs (harps) are available for tissue slice studies. Anchors are available with thread spacing (Lycra) at 1.0, 1.5, and 2.0 mm.
Both chambers require a platform (PM-1, PH-1, or P-1) and Series 20 stage adapter to complete the assembly for mounting on a microscope. The chamber floor consists of a 22 x 40 mm coverslip.
Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.
An application video is available for this product through the JoVE website!
You can view our selection of videos and access full length videos here.
Large volume open diamond bath recording chamber that can accommodate large specimens such as slice preparations.
Applications include patch clamp, intracellular/extracellular recording, and imaging
Can be used with tissue slices or cell cultured coverslips