Specialized Tools for Electrophysiology and Cell Biology Research
- Customer Service: 800-232-2380
- Sales: 833-668-8632
(RC-21BRW) Open Round Bath Imaging Chamber
Product Summary
This open bath recording chamber features a wide round aperture for maximum imaging area.
- Designed for physiological measurements on cell cultured coverslips
- Uses 25 mm diameter coverslip as the chamber bottom
- Quiet operation
- Broad imaging area
- Wicked solution removal from bath
- Can be used with upright or inverted microscopes
The RC-21BRW is an open bath recording chamber using a 25 mm diameter round coverslip as the chamber bottom. The wide, round aperture of the RC-21BRW maximizes the viewing area on the coverslip and enables excellent access for electrodes. Constant solution level and quiet operation are assured with the use of a wick between the bath and suction well. Bath volume is 240 µl per mm solution height.
The low profile design of the RC-21BRW is ideal for use with large diameter objectives and will accommodate either upright or inverted microscopes. The large imaging area (17 mm diameter) increases the likelihood of viewing desired cell features located around the periphery of the chamber.
RC-21BRW chambers require a platform (PM-2, PH-2, P-2) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.
Each chamber includes a pack of #1 thickness glass coverslips (CS-25R, 25 mm diameter, 100/pkg) and 10 ft of PE-160 tubing.
An application video is available for this product through the JoVE website! You can view our selection of videos and access full length videos here.
| Material | Polycarbonate |
|---|---|
| Bath dimension (D x H) | 17.4 mm x 2.5 mm |
| Volume, by depth | 240 μl/mm |
| Bottom coverglass | 25 mm round |
| Minimum access angle (from side to center of bath) | 16° |
| Injection port (ID x OD) |
0.91 x 0.56 mm, stainless steel |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
| Output tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
| Order No. | Model No. | Product Description | USA Price | Order. |
|---|---|---|---|---|
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
References
References
- Na+,K+,2Cl– Cotransport and Intracellular Chloride Regulation in Rat Primary Sensory Neurons: Thermodynamic and Kinetic Aspects
Rocha-González HI, Mao S, Alvarez-Leefmans FJ
J Neurophysiol. 2008 Jul;100(1):169-84. Epub 2008 Apr 2 - Simultaneous Measurement of Water Volume and pH in Single Cells Using BCECF and Fluorescence Imaging Microscopy
Francisco J. Alvarez-Leefmans, Jose J. Herrera-Perez, Martin S. Marquez,y and Victor M. Blanco
Biophysical Journal Volume 90 January 2006 608.618 - Modulation of Monocarboxylic Acid Transporter-1 Kinetic Function by the cAMP Signaling Pathway in Rat Brain Endothelial Cells*
Smith JP, Drewes LR
J Biol Chem. 2006 Jan 27;281(4):2053-60. Epub 2005 Nov 21
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chamber | ||||
| 64-0227 | RC-21BRW | Open bath chamber, 100 µl volume, 25 mm round coverslip | Login | Login |
| Platforms | ||||
| 64-1561. | PM-2 | PM-2 Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
| 64-0285 | PH-2 | PH-2 Platform for Series 20 chambers, heater | Login | Login |
| Replacement Consumables | ||||
| 64-0705 | CS-25R | Round cover glass, #1 thickness, 25 mm, 100 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| Replacement Parts | ||||
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |