Specialized Tools for Electrophysiology and Cell Biology Research

(RC-21BRW) Open Round Bath Imaging Chamber

Product Summary

This open bath recording chamber features a wide round aperture for maximum imaging area.

  • Designed for physiological measurements on cell cultured coverslips
  • Uses 25 mm diameter coverslip as the chamber bottom
  • Quiet operation
  • Broad imaging area
  • Wicked solution removal from bath
  • Can be used with upright or inverted microscopes

The RC-21BRW is an open bath recording chamber using a 25 mm diameter round coverslip as the chamber bottom. The wide, round aperture of the RC-21BRW maximizes the viewing area on the coverslip and enables excellent access for electrodes. Constant solution level and quiet operation are assured with the use of a wick between the bath and suction well. Bath volume is 240 µl per mm solution height.

The low profile design of the RC-21BRW is ideal for use with large diameter objectives and will accommodate either upright or inverted microscopes. The large imaging area (17 mm diameter) increases the likelihood of viewing desired cell features located around the periphery of the chamber.

RC-21BRW chambers require a platform (PM-2PH-2P-2) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.
 

 

Each chamber includes a pack of #1 thickness glass coverslips (CS-25R, 25 mm diameter, 100/pkg) and 10 ft of PE-160 tubing.


An application video is available for this product through the JoVE website! You can view our selection of videos and access full length videos here.

Material Polycarbonate
Bath dimension (D x H) 17.4 mm x 2.5 mm
Volume, by depth 240 μl/mm
Bottom coverglass 25 mm round
Injection port
(ID x OD)
0.91 x 0.56 mm, stainless steel
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Order No. Model No. Product Description USA Price Order
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Na+,K+,2Cl– Cotransport and Intracellular Chloride Regulation in Rat Primary Sensory Neurons: Thermodynamic and Kinetic Aspects
    Rocha-González HI, Mao S, Alvarez-Leefmans FJ
    J Neurophysiol. 2008 Jul;100(1):169-84. Epub 2008 Apr 2
  2. Simultaneous Measurement of Water Volume and pH in Single Cells Using BCECF and Fluorescence Imaging Microscopy
    Francisco J. Alvarez-Leefmans, Jose J. Herrera-Perez, Martin S. Marquez,y and Victor M. Blanco
    Biophysical Journal Volume 90 January 2006 608.618
  3. Modulation of Monocarboxylic Acid Transporter-1 Kinetic Function by the cAMP Signaling Pathway in Rat Brain Endothelial Cells*
    Smith JP, Drewes LR
    J Biol Chem. 2006 Jan 27;281(4):2053-60. Epub 2005 Nov 21

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
Chamber
64-0227 RC-21BRW Open bath chamber, 100 µl volume, 25 mm round coverslip Login Login
Platforms
64-1561 PM-2 PM-2 Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-0285 PH-2 PH-2 Platform for Series 20 chambers, heater Login Login
64-0278 P-2 P-2 Platform for Series 20 chambers, non-heater Login Login
Replacement Consumables
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Temperature Control
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories
64-1940 DWV Dedicated Workstation Vacuum Login Login
Replacement Parts
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login

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