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Specialized Tools for Electrophysiology and Cell Biology Research

(SC-20) In-line Heater/Cooler

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Product Summary

This dual in-line solution heater/cooler extends the temperature range for controlled perfusion studies of both coverslip mounted cells and anchored tissue thin slices.

 

  • Heats or cools perfusate*
  • Compatible with all Warner Series 20 chambers
  • Requires a CL-100 or CL-200A Bipolar Temperature Controller and LCS-1 Heat Exchanger
  • Versatile and simple to use thermal control device

 

In-line solution heating has proven to be one of the most effective methods of maintaining the temperature of perfusion solutions. The SC-20 Dual In-line Solution Heater/Cooler utilizes Peltier thermoelectric technology to regulate temperature both above and below ambient levels.

The SC-20 is designed to thermally regulate eith3re one or two solutions at the same temperature. Temperature regulation is provided by the CL-200A or CL-100 Bipolar Temperature controller.

Solution temperatures can be maintained at 0°C at flow rates of 2 ml/min., 5°C at 5 ml/min., or as high as 50°C at 5 ml/min.

An integral water jacket is used to remove excess heat from the SC-20 and the LCS-1 is specifically designed to provide this service.

The SC-20 can be used with either one or two discrete perfusate solutions, or with a solution/gas combination. When coupled with a PHC Series Imaging Chamber Heater/Cooler Jacket, the SC-20 provides an effective means of temperature control in the chamber, even under slow-flow conditions.

* Be advised that saturation limits for many ionic compounds are decreased at lower temperatures and that this is not a property of the SC-20 or other Warner apparatus.

Each heater/cooler is supplied with a TA-29 thermistor cable assembly for monitoring the bath temperature.

Minimum Temperature 0°C at 2 mL/min
maximum flow
Maximum Temperature 50°C
Maximum Flow
Rate at 5°C		 5 mL/min
Accuracy ± 0.1°C
Internal Dead Volume 330 µL
Controller Model CL-100
Bipolar Controller
Perfusion Lines Type 316 Stainless Steel
0.032 ID x 0.062 OD
Water Jacket Ports Type 316 Stainless Steel
0.12 ID x 0.147 OD
Body Physical
Dimensions		 21 mm diameter x
165 mm long
Weight 109 grams
Cable Length 1.9 meters
Connector Type 15 pin Male D
Warranty 1 year

Infrared Image

In-line Heater/Cooler (SC-20)

 
QE-1HC Quick Exchange Heated / Cooled platform with SC-20: cooling with perfusion.
Conditions:
Ambient Temperature 21.5C
Relative Humidity 62.3%
CL-200 Temperature Controller:
Set Temperature SC-20 0.0C
QE-1HV Plate Set Temperature 0.0C
Flow Rate 2ml/min.

References

References

  1. The Effect of Thermal Acclimation on Action Potentials and Sarcolemmal K+ Channels from Pacific Bluefin Tuna Cardiomyocytes
    Galli GL, Lipnick MS, Block BA
    Am J Physiol Regul Integr Comp Physiol. 2009 Aug;297(2):R502-9
  2. Temporal Dynamics of Neuronal Activation by Channelrhodopsin-2 and TRPA1 Determine Behavioral Output in Drosophila Larvae
    Pulver SR, Pashkovski SL, Hornstein NJ, Garrity PA, Griffith LC
    J Neurophysiol. 2009 Jun;101(6):3075-88
  3. Pore region of TRPV3 ion channel is specifically required for heat activation
    Grandl J, Hu H, Bandell M, Bursulaya B, Schmidt M, Petrus M, Patapoutian A
    Nat Neurosci. 2008 Sep;11(9):1007-13
  4. Identification of molecular determinants of channel gating in the transient receptor potential box of vanilloid receptor I
    Valente P, García-Sanz N, Gomis A, Fernández-Carvajal A, Fernández-Ballester G, Viana F, Belmonte C, Ferrer-Montiel A
    FASEB J. 2008 Sep;22(9):3298-309
  5. An internal thermal sensor controlling temperature preference in Drosophila
    Hamada FN, Rosenzweig M, Kang K, Pulver SR, Ghezzi A, Jegla TJ, Garrity PA
    Nature. 2008 Jul 10;454(7201):217-20
  6. The TRPV3 mutation associated with the hairless phenotype in rodents is constitutively active
    Xiao R, Tian J, Tang J, Zhu MX
    Cell Calcium. 2008 Apr;43(4):334-43
  7. Highly Ca2+-selective TRPM channels regulate IP3-dependent oscillatory Ca2+ signaling in the C. elegans intestine
    Xing J, Yan X, Estevez A, Strange K
    J Gen Physiol. 2008 Mar;131(3):245-55
  8. Divergent Biophysical Defects Caused by Mutant Sodium Channels in Dilated Cardiomyopathy With Arrhythmia
    Nguyen TP, Wang DW, Rhodes TH, George AL Jr
    Circ Res. 2008 Feb 15;102(3):364-71
  9. A Role of the Transient Receptor Potential Domain of Vanilloid Receptor I in Channel Gating
    García-Sanz N, Valente P, Gomis A, Fernández-Carvajal A, Fernández-Ballester G, Viana F, Belmonte C, Ferrer-Montiel A
    J Neurosci. 2007 Oct 24;27(43):11641-50
  10. In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space
    Thorne RG, Nicholson C
    Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5567-72
  11. Diurnal and estradiol-dependent changes in gonadotropin-releasing hormone neuron firing activity
    Christian CA, Mobley JL, Moenter SM
    Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15682-7
  12. Excitatory monocyte chemoattractant protein-1 signaling is up-regulated in sensory neurons after chronic compression of the dorsal root ganglion
    White FA, Sun J, Waters SM, Ma C, Ren D, Ripsch M, Steflik J, Cortright DN, Lamotte RH, Miller RJ
    Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14092-7
  13. Subtype-Specific GABA Transporter Antagonists Synergistically Modulate Phasic and Tonic GABAA Conductances in Rat Neocortex
    Keros S, Hablitz JJ
    J Neurophysiol. 2005 Sep;94(3):2073-85
  14. High-Concentration Rapid Transients of Glutamate Mediate Neural-Glial Communication via Ectopic Release
    Matsui K, Jahr CE, Rubio ME
    J Neurosci. 2005 Aug 17;25(33):7538-47
  15. Biphasic Currents Evoked by Chemical or Thermal Activation of the Heat-gated Ion Channel, TRPV3*
    Chung MK, Güler AD, Caterina MJ
    J Biol Chem. 2005 Apr 22;280(16):15928-41

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
64-0353 SC-20 Solution heater / cooler, dual channel Login Login
Accessories and Replacement Parts
64-0352 CL-100 Bipolar temperature controller Login Login
64-1922 LCS-1 Liquid Cooling Device Login Login
64-0354 PHC-1 Heater/Cooler jacket for Series 20 chambers (not compatible with inverted microscopes) Login Login
64-0355 PHC-2 Heater/Cooler jacket for diamond bath Series 20 chambers Login Login

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