Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-27) Large Rectangular Open Bath Chamber
Product Summary
A large bath chamber suitable for studies requiring smooth solution flow with a large viewing area.
- Accommodates large tissue sections or brain slices
- Large rectangular bath
- Large optical imaging area
- Can be used with either upright or inverted microscopes
- Slice anchors available
The RC-27 is a large bath chamber ideal for large tissue or brain slice studies. The large imaging area increases the likelihood of viewing desired features in the angled sides of the chamber facilitate electrode placement.
The chamber floor consists of a 24 x 50 mm rectangular coverslip and the bath area is 12 x 24mm. these dimensions are useful for organotypic slice culture studies. An adjustable spreader plate at the bath input facilitates uniform solution flow. The working volume of the RC-27 is 413 ul per mm of solution height. The chamber is equipped with a stainless steel aspiration tube together with a clamp.
Designed for brain or tissue slice studies, several optional slice anchors are offered with Lycra thread spacings of 1.0, 1.5, or 2.0 mm. An anchor kit is also available containing slice anchors (1 each) in each available thread spacing.
The RC-27 requires a platform (PM-6 or PH-6) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.
Each chamber is supplied with a package of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, 50/pkg) and 10 ft of PE-160 tubing. Available slice anchors sold separately.
| Material | Polycarbonate |
|---|---|
| Bath dimensions (L x W x H) |
38 x 12.5 x 4 mm |
| Volume, by depth | 413 μl/mm |
| Minimum access angle (from side to center of bath) | 24° |
| Bottom coverglass | 24 x 50 mm |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order. |
|---|---|---|---|---|
| 64-0708 | CS-22/50 | Rectangular cover glass, #1 thickness, 22 x 50 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
References
References
- Effects of Oleic Acid on Distinct Populations of Neurons in the Hypothalamic Arcuate Nucleus Are Dependent on Extracellular Glucose Levels,
R. Wang1, C. Cruciani-Guglielmacci2, S. Migrenne2, C. Magnan2, V. E. Cotero1 and V. H. Routh1,
J Neurophysiol 95: 1491-1498, 2006. First published November 23, 2005 - Reactive oxygen species formation in the transition to hypoxia in skeletal muscle,
Li Zuo and Thomas L. Clanton,
Am J Physiol Cell Physiol 289: C207-C216, 2005
Related Products
Related Files
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chamber | ||||
| 64-0238 | RC-27 | Very large bath recording chamber | Login | Login |
| Platforms | ||||
| 64-1527 | PM-6 | PM-6 Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
| 64-0289 | PH-6 | PH-6 Platform for Series 20 chambers, heater | Login | Login |
| Slice Anchors (Harps) | ||||
| 64-0267 | SHD-27KIT | Slice anchor kit for RC-27, stainless steel | Login | Login |
| 64-0194 | SHD-27H/10 | Slice anchor for RC-27, 1.0 mm thread spacing, stainless steel | Login | Login |
| 64-0193 | SHD-27H/15 | Slice anchor for RC-27, 1.5 mm thread spacing, stainless steel | Login | Login |
| 64-0192 | SHD-27H/2 | Slice anchor for RC-27, 2.0 mm thread spacing, stainless steel | Login | Login |
| Replacement Consumables | ||||
| 64-0709 | CS-24/50 | Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| Replacement Parts | ||||
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |