Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-27D, RC-27LD) Ultra-quiet Imaging Chambers for Slice Studies
Product Summary
The RC-27D and RC-27LD are designed for applications requiring ultra quiet solution delivery.
- Designed for tissue slice studies
- RC-27LD includes a slice support
- Sloped input eliminates vibration associated with solution delivery
- Alows multiplexed solution exchange without switching artifacts
The large imaging field in the RC-27D and RC-27LD allows for maximal observation of biological specimens with the addition of a sloped input. The advantages of the sloped inflow channel includes smooth and "ultra-quiet" solution delivery.
The sloped input allows I-V type infusion lines to deliver drops at a fixed rate without the transfer of noise to the specimen field. In addition, multiplexed solution delivery is now possible without the potential for solution switching artifacts. This technology provides the capability of ultra-quiet solution exchange without transferring noise to either the system or to the image.
Ultra-quiet solution delivery when measuring from tissue slices is provided by the inclusion of a slice support in the RC-27LD chamber. Slices are supported 0.5 mm above the chamber floor. Support threads are made of Lycra and thread spacing 0.5 mm.
The RC-27D requires a PM-7 platform while the RC-27LD requires a PM-7D platform. The increased depth of the PM-7D platform permits use of the associated RC-27LD slice support. Both platforms are compatible our Series 20 stage adapters which complete the assembly for mounting onto a microscope.
Both chambers are supplied with a MAG-2 magnetic clamp for suction tubes, a magnetic perfusion tube holder, a package of #2 thickness glass coverslips (CS-24/60), 40/pkg, and 10 feet of PE-160 polyethylene tubing. The RC-27LD also includes a slice support. Slice anchors are sold separately.
| Material | Polycarbonate |
|---|---|
| Bath dimensions (L x W x H) |
38 x 18 x 2.5 mm |
| Outer dimensions (L x W x H) |
59.9 x 25.4 x 4.2 mm |
| Volume, by depth | 560 µl/mm |
| Minimum access angle (from side to center of bath) | 16° |
| Bottom coverglass | 24 x 60 mm |
| Order No. | Model No. | Product Description | USA Price | Order. |
|---|---|---|---|---|
| 64-0710 | CS-24/60 | Rectangular cover glass, #2 thickness, 24 x 60 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-1551 | SS-4-500H | Slice support, 500 um thread spacing, parallel to flow, for RC-27D, RC-27LD drip chambers | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
References
References
- Cholecystokinin Activates Orexin/Hypocretin Neurons through the Cholecystokinin A Receptor
Tsujino N, Yamanaka A, Ichiki K, Muraki Y, Kilduff TS, Yagami K, Takahashi S, Goto K, Sakurai T
J Neurosci. 2005 Aug 10;25(32):7459-69 - Chronic Cocaine Administration Switches Corticotropin-Releasing Factor2 Receptor-Mediated Depression to Facilitation of Glutamatergic Transmission in the Lateral Septum
Liu J, Yu B, Orozco-Cabal L, Grigoriadis DE, Rivier J, Vale WW, Shinnick-Gallagher P, Gallagher JP
J Neurosci. 2005 Jan 19;25(3):577-83
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chambers | ||||
| 64-1548 | RC-27D | Ultra Quiet Imaging Chamber, large bath, IV drip type | Login | Login |
| 64-1532 | RC-27LD | Ultra Quiet Imaging Chamber, very large bath, IV drip type, with slice support | Login | Login |
| Platforms | ||||
| 64-1529 | PM-7 | PM-7 Platform for RC-27D chambers, magnetic clamps, heated | Login | Login |
| 64-1530 | PM-7D | PM-7D Platform for RC-27LD chambers, magnetic clamps, heated | Login | Login |
| Slice Anchors (Harps) | ||||
| 64-0268 | SHD-27LKIT | Slice anchor kit for RC-27L, RC-27D, RC-29, stainless steel | Login | Login |
| 64-0256 | SHD-27LH/10 | Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, stainless steel | Login | Login |
| 64-0257 | SHD-27LH/15 | Slice anchor for RC-27L, RC-27D, RC-29, 1.5 mm thread spacing, stainless steel | Login | Login |
| 64-0258 | SHD-27LH/2 | Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, stainless steel | Login | Login |
| Slice Anchors (Harps) - Plastic Frame | ||||
| 64-0259 | SHD-27LP/10 | Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, plastic frame | Login | Login |
| 64-0261 | SHD-27LP/2 | Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, plastic frame | Login | Login |
| Replacement Consumables | ||||
| 64-0710 | CS-24/60 | Rectangular cover glass, #2 thickness, 24 x 60 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| Replacement Parts | ||||
| 64-1450 | CPS-27L | Perfusion spreader plate for RC-27L | Login | Login |
| 64-1551 | SS-4-500H | Slice support, 500 um thread spacing, parallel to flow, for RC-27D, RC-27LD drip chambers | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |