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Specialized Tools for Electrophysiology and Cell Biology Research

(SH-27B, SF-28) Single Inline Solution Heaters

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Product Summary

In-line solution heaters are the most direct way to warm solutions flowing into a Series 20 Imaging and Recording Chamber.

 

  • Small size allows placement close to imaging and recording recording so as to minimize convective heat losses
  • Single line in-line solution heaters include T1 thermister embedded within aluminum housing for feedback control
  • Designed to operate with TC-344C or TC-324C temperature controllers
  • Output temperature - ambient to 65°C

In-line solution heating is the simplest and most effective method of warming perfusion solutions. The heater is connected to the chamber with a short length of tubing such that the warmed perfusate flows directly into the chamber bath. Depending on bath volume and other factors, in-line solution heating alone by it self may be sufficient for many applications.

Single Inline Solution Heaters (SH-27B, SF-28)

 

In-line solution heaters from Warner Instruments are used to warm perfusate solutions as they flow into a chamber (such as our Imaging and Recording Chambers). Their small size allows for placement close to the chamber input allowing the perfusate to be warmed immediately prior to entry. The SH-27B is our rapid flow in-line solution heater. It is ideal for perfusion flows up to 10 ml/min while the SF-28 is recommended for applications requiring solution flow rates of less than 2 ml/min.

Single Inline Solution Heaters (SH-27B, SF-28)

 

Temperature control is maintained by an internal feedback thermistor (i.e. T1 on the heater controller) which senses the temperature at the outflow end of the heater. This sensing thermistor is located outside of the solution path and is therefore not subjected to corrosive salts or careless cleaning.

Each heater is also supplied with a Model TA-29 monitor thermistor cable assembly (as shown on the right) which allows for monitoring the actual bath temperature (T2 output on the heater controller). Solutions are gradually warmed as they flow from input to output resulting in minimal outgassing. Heater input and output lines accept standard 1/16" OD (PE-160) tubing.

Heater Resistance 10Ω
Voltage Requirement Variable 0 to 12 VDC max.
Maximum Temperature 65°C
Internal Dead Volume 260 μl
Maximum Flow Rate at 37°C
SF-28 2 ml/min
SH-27B 10 ml/min
Physical Dimensions
Body 12.5 mm x 12.5 cm (D x L)
Cable 1.9 m
Bath thermistor dimensions 0.85 x 4.6 mm
Warranty 1 year
Temperature of 37°C can be maintained at ± 0.2°C under following conditions:
Condition A Solution temperature at input (nominally 21°C) varies no more than ±10%
Condition B Solution flow rate varies no more than 25%

Infrared Image

Single Inline Solution Heaters (SH-27B, SF-28)

 
QE-1 Quick Exchange Platform with SH-27B In-Line Heater: heating with perfusion.
Conditions:
Ambient Temperature 21.5C
Relative Humidity 62.3%
TC-344B Temperature Controller:
QE-1 Set Temperature 38C
SH-27B Set Temperature 40C
Flow Rate 2 ml/min.

 

Last Revision 1.9.13

 

Order No. Model No. Product Description USA Price Order.
64-0107 TA-29 Cable with bead thermistor Login Login
64-0108 TA-30 Cable with glass thermistor Login Login
64-1555 SHH-1 Holder for SH-27B and SF-28 inline solution heaters Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login

References

References

  1. Knockdown of Stromal Interaction Molecule 1 Attenuates Store-operated Ca2+ Entry and Ca2+ Responses to Acute Hypoxia in Pulmonary Arterial Smooth Muscle
    Lu W, Wang J, Peng G, Shimoda LA, Sylvester JT
    Am J Physiol Lung Cell Mol Physiol. 2009 Jul;297(1):L17-25
  2. Enhancement of inhibitory synaptic transmission in large aspiny neurons after transient cerebral ischemia
    Li Y, Lei Z, Xu ZC
    Neuroscience. 2009 Mar 17;159(2):670-81
  3. Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia
    Lu W, Wang J, Shimoda LA, Sylvester JT
    Am J Physiol Lung Cell Mol Physiol. 2008 Jul;295(1):L104-13
  4. Shear stress regulates aquaporin-5 and airway epithelial barrier function
    Sidhaye VK, Schweitzer KS, Caterina MJ, Shimoda L, King LS
    Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3345-50
  5. Synaptic Vesicle Mobility in Mouse Motor Nerve Terminals with and without Synapsin
    Gaffield MA, Betz WJ
    J Neurosci. 2007 Dec 12;27(50):13691-700
  6. Light-Evoked Calcium Responses of Isolated Melanopsin-Expressing Retinal Ganglion Cells
    Hartwick AT, Bramley JR, Yu J, Stevens KT, Allen CN, Baldridge WH, Sollars PJ, Pickard GE
    J Neurosci. 2007 Dec 5;27(49):13468-80
  7. Ca2+ signaling in hypoxic pulmonary vasoconstriction: effects of myosin light chain and Rho kinase antagonists
    Wang J, Weigand L, Foxson J, Shimoda LA, Sylvester JT
    Am J Physiol Lung Cell Mol Physiol. 2007 Sep;293(3):L674-85
  8. Dopamine D1/5 Receptor-Mediated Long-Term Potentiation of Intrinsic Excitability in Rat Prefrontal Cortical Neurons: Ca2+-Dependent Intracellular Signaling
    Long Chen, Joseph D. Bohanick, Makoto Nishihara, Jeremy K. Seamans and Charles R. Yang
    J Neurophysiol. 2007 Mar;97(3):2448-64.
  9. Gasping Activity In Vitro: A Rhythm Dependent on 5-HT2A Receptors
    Andrew K. Tryba, Fernando Pen˜a, and Jan-Marino Ramirez
    The Journal of Neuroscience, March 8, 2006 • 26(10):2623–2634 • 2623
  10. The Level and Integrity of Synaptic Input Regulates Dendrite Structure
    Staci A. Sorensen and Edwin W. Rubel
    The Journal of Neuroscience, February 1, 2006 • 26(5):1539 –1550
  11. Real-Time 2-Photon Imaging of Mitochondrial Function in Perfused Rat Hearts Subjected to Ischemia/Reperfusion
    Matsumoto-Ida M, Akao M, Takeda T, Kato M, Kita T
    Circulation. 2006 Oct 3;114(14):1497-503
  12. Neurofilament Polymer Transport in Axons
    Yanping Yan1, and Anthony Brown1
    The Journal of Neuroscience, July 27, 2005, 25(30):7014-7021
  13. Disturbed Ca2+ signaling and apoptosis of medium spiny neurons in Huntington's disease
    Tie-Shan Tang, Elizabeth Slow, Vitalie Lupu, Irina G. Stavrovskaya , Mutsuyuki Sugimori, Rodolfo Llinás, Bruce S. Kristal, Michael R. Hayden, and Ilya Bezprozvanny
    PNAS | February 15, 2005 | vol. 102 | no. 7 | 2602-2607
  14. Orexin-A-induced Ca2+ entry: evidence for involvement of trpc channels and protein kinase C regulation
    Kim P. Larsson, Hanna M. Peltonen, Genevieve Bart, Lauri M. Louhivuori, Annika Penttonen, Miia Antikainen, Jyrki P. Kukkonen, and Karl E. O. Åkerman,
    J. Biol. Chem., Vol. 280, Issue 3, 1771-1781, January 21, 2005
  15. Four-Dimensional Migratory Coordinates of GABAergic Interneurons in the Developing Mouse Cortex
    Ang ES Jr, Haydar TF, Gluncic V, Rakic P
    J Neurosci. 2003 Jul 2;23(13):5805-15

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
64-0102 SH-27B In-line solution heater Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login
Accessories and Replacement Parts
64-1555 SHH-1 Holder for SH-27B and SF-28 inline solution heaters Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0108 TA-30 Cable with glass thermistor Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
Temperature Controllers
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login

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