Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-24N) Fast Exchange Open Diamond Bath
Product Summary
The RC-24N allows very fast solution exchange for a variety of applications. This recording chamber features the smallest bath volume of the open style chambers.
- Designed for physiological measurements of cell cultured coverslips
- Applications include patch clamp, intracellular/extracellular recordings, and imaging
- Diamond shaped bath provides laminar solution flow
- Very small bath volume facilitates very fast solution exchange
The RC-24N chamber design is taken from the RC-24 and improves the flow characteristics by providing a linear path. The bath volume in this model is among the smallest of the open style chambers. This enables very fast solution exchange for a variety of applications, including patch clamp and electrophysiology studies. The small bath volume is especially useful when using drugs in small quantities.
An agar bridge well for reference electrodes is provided, as is an aspiration well. The aspiration well is equipped with a stainless steel aspiration tube (1.67 mm OD) together with a tube clamp. The clamp insures that the adjustable suction tube will not move once the height is set by the user. The floor of this chamber is formed from a 22 mm x 40 mm rectangular coverslip.
The RC-24N requires a platform (PM-1, PH-1 or P-1) and Series 20 stage adapter to complete the assembly for mounting on a microscope.
Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.
| Material | Polycarbonate |
|---|---|
| Bath dimensions (L x W x H) |
15 x 6 x 3.8 mm |
| Volume, by depth | 50 μl/mm |
| Minimum access angle (from side to center of bath) | 28° |
| Bottom coverglass | 22 x 40 mm |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order. |
|---|---|---|---|---|
| 64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
References
Videos
- Perfusion and Aspiration Techniques
Warner Instruments
YouTube 2012
References
- Orexin-A-induced Ca2+ Entry EVIDENCE FOR INVOLVEMENT OF TRPC CHANNELS AND PROTEIN KINASE C REGULATION*,
Kim P. Larsson, Hanna M. Peltonen, Genevieve Bart, Lauri M. Louhivuori, Annika Penttonen, Miia Antikainen, Jyrki P. Kukkonen, and Karl E. O. Åkerman,
J. Biol. Chem., Vol. 280, Issue 3, 1771-1781, January 21, 2005 - Stimulation of astrocyte Na+/H+ exchange activity in response to in vitro ischemia depends in part on activation of ERK1/2,
Douglas B. Kintner, Andy Look, Gary E. Shull, and Dandan Sun,
Am J Physiol Cell Physiol 289: C934-C945, 2005 - Decreased Neuronal Death in Na+/H+ Exchanger Isoform 1-Null Mice after In Vitro and In Vivo Ischemia,
Jing Luo, Hai Chen, Douglas B. Kintner, Gary E. Shull, and Dandan Sun,
The Journal of Neuroscience, December 7, 2005, 25(49):11256-11268
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chamber | ||||
| 64-0381 | RC-24N | Fast exchange recording chamber, narrow bath, 50 µl volume | Login | Login |
| Platforms | ||||
| 64-1526 | PM-1 | PM-1 Platform for Series 20 chambers, heated, magnetic clamps | Login | Login |
| 64-0284 | PH-1 | PH-1 Platform for Series 20 chambers, heater | Login | Login |
| Replacement Consumables | ||||
| 64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Replacement Parts | ||||
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |