Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-25, RC-25F) Open Diamond Bath Imaging Chambers for Round Coverslips
Product Summary
Open diamond bath recording chambers designed for round coverslips.
- Designed for physiological measurements of cell cultured coverslips
- Applications include patch clamp, intracellular/extracellular recordings, and imaging
- Uses 12 and 15 mm diameter round coverslips as the chamber floor
- Diamond shaped bath provides laminar solution flow
- Small bath volume facilitates fast solution exchange
The design of the RC-25 and RC-25F chambers incorporates a diamond-shaped bath which has been shown to produce laminar flow. Since the bath volumes of these chambers is small (90 and 133 ul/mm for the RC-25 and RC-25F, respectively), fluid exchange times are measured in seconds even when flow rates are less than 1 ml/min.
RC-25 Chamber
The RC-25 works equally well with either 12 or 13 mm diameter coverslips while the RC-25F uses 15 mm coverslips. In general, these coverslips are used to form the bottom of the chamber bath. NOTE: These chambers are not designed for insertion of coverslips from the top.
The RC-25 requires a platform (PM-3, PH-3 or P-3) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.
The RC-25 is supplied with a package of #1.5 thickness glass coverslips (CS-12R, 12 mm diameter, 100/pkg) and 10 ft of PE-160 tubing.
RC-25F Chamber
The RC-25F is similar in design to the RC-25 but uses 15 mm coverslips. In general, these coverslips are used to form the bottom of the chamber bath. NOTE: These chambers are not designed for insertion of coverslips from the top.
The RC-25F requires a platform (PM-4, PH-4 or P-4) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.
The RC-25F is supplied with a package of #1.5 thickness glass coverslips (CS-15R, 15 mm diameter, 100/pkg) and 10 ft of PE-160 tubing.
| RC-25 | RC-25F | |
|---|---|---|
| Material | polycarbonate | polycarbonate |
| Chamber dimensions (L x W x H) |
39.6 x 22.0 x 4.15 mm | 39.6 x 22.0 x 4.15 mm |
| Bath dimensions (L x W x H) |
25 x 12 x 4.1 mm | 24 x 13 x 4.1 mm |
| Volume, by depth | 90 μl/mm | 133 μl/mm |
| Minimum access angle (from side to center of bath) | 36° | 33° |
| Bottom coverglass | 12 mm round | 15 mm round |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) | 1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order. |
|---|---|---|---|---|
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-0712 | CS-12R15 | Round cover glass, #1.5 thickness, 12 mm, 100 pack | Login | Login |
| 64-0713 | CS-15R15 | Round cover glass, #1.5 thickness, 15 mm, 100 pack | Login | Login |
References
References
- Zonisamide block of cloned human T-type voltage-gated calcium channels
Matar N, Jin W, Wrubel H, Hescheler J, Schneider T, Weiergräber M
Epilepsy Res. 2009 Feb;83(2-3):224-34. Epub 2009 Jan 4 - Structural and Functional Analysis of the HCV p7 Protein
Saint N, Montserret R, Chipot C, Penin F
Methods Mol Biol. 2009;510:125-43 - Extracellular nucleotides stimulate Cl– currents in biliary epithelia through receptor-mediated IP3 and Ca2+ release
Dutta AK, Woo K, Doctor RB, Fitz JG, Feranchak AP
Am J Physiol Gastrointest Liver Physiol. 2008 Nov;295(5):G1004-15. Epub 2008 Sep 11 - Fluid flow induces mechanosensitive ATP release, calcium signalling and Cl? transport in biliary epithelial cells through a PKC?-dependent pathway
Woo K, Dutta AK, Patel V, Kresge C, Feranchak AP
J Physiol. 2008 Jun 1;586(Pt 11):2779-98. Epub 2008 Apr 3 - Activation of Cl– Channels by Human Chorionic Gonadotropin in Luteinized Granulosa Cells of the Human Ovary Modulates Progesterone Biosynthesis
Olivero P, Leiva-Salcedo E, Devoto L, Stutzin A
Endocrinology. 2008 Sep;149(9):4680-7. Epub 2008 May 22 - State-dependent calcium mobilization by urotensin-II in cultured human endothelial cells
Brailoiu E, Jiang X, Brailoiu GC, Yang J, Chang JK, Wang H, Dun NJ
Peptides. 2008 May;29(5):721-6. Epub 2008 Jan 31 - Imaging pHluorin-Based Probes at Hippocampal Synapses
Royle SJ, Granseth B, Odermatt B, Derevier A, Lagnado L
Methods Mol Biol. 2008;457:293-303 - Role of Angiotensin II Type 1A Receptor Phosphorylation, Phospholipase D, and Extracellular Calcium in Isoform-specific Protein Kinase C Membrane Translocation Responses
Aleksandra Policha, Noriko Daneshtalab, Lina Chen, Lianne B. Dale, Christophe Altier, Houman Khosravani, Walter G. Thomas, Gerald W. Zamponi, and Stephen S. G. Ferguson
J. Biol. Chem., Vol. 281, Issue 36, 26340-26349, September 8, 2006 - A novel role for protein phosphatase 2A in receptor-mediated regulation of the cardiac sarcolemmal Na+/H+ exchanger NHE1
Andrew K. Snabaitis, Richard D’Mello, Semjidmaa Dashnyam, and Metin Avkiran1
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 281, NO. 29, pp. 20252–20262, July 21, 2006 - Translocation of Synaptically Connected Interneurons across the Dentate Gyrus of the Early Postnatal Rat Hippocampus
Yury M. Morozov, Albert E. Ayoub, and Pasko Rakic
The Journal of Neuroscience, May 10, 2006 • 26(19):5017–5027 - Molecular and Morphological Heterogeneity of Neural Precursors in the Mouse Neocortical Proliferative Zones
Jonathan S. Gal, Yury M. Morozov, Albert E. Ayoub, Mitali Chatterjee,1 Pasko Rakic, and Tarik F. Haydar
The Journal of Neuroscience, January 18, 2006 • 26(3):1045–1056 - Cyclic nucleotide regulation of store-operated Ca2 influx in airway smooth muscle
Binnaz Ay, Adeyemi Iyanoye, Gary C. Sieck, Y. S. Prakash and Christina M. Pabelick
Am J Physiol Lung Cell Mol Physiol 290:278-283, 2006 - Store-operated Ca2 Influx in Airway Smooth Muscle Interactions between Volatile Anesthetic and Cyclic Nucleotide Effects
Y. S. Prakash, M.D., Ph.D., Adeyemi Iyanoye, M.D., Binnaz Ay, M.D., Gary C. Sieck, Ph.D., Christina M. Pabelick, M.D.
Anesthesiology 2006; 105:976–83 - Integration of K+ and Cl currents regulate steady-state and dynamic membrane potentials in cultured rat microglia
Evan W. Newell, Lyanne C. Schlichter
The Journal of Physiology Volume 567 Issue 3 Page 869-890, September 2005 - Four-Dimensional Migratory Coordinates of GABAergic Interneurons in the Developing Mouse Cortex
Ang ES Jr, Haydar TF, Gluncic V, Rakic P
J Neurosci. 2003 Jul 2;23(13):5805-15
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chambers | ||||
| 64-0232 | RC-25 | Round coverslip recording chamber for 12 or 13 mm round coverslips | Login | Login |
| 64-0233 | RC-25F | Round coverslip recording chamber for 15 mm round coverslips | Login | Login |
| Platforms for RC-25 | ||||
| 64-1562 | PM-3 | PM-3 Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
| 64-0286 | PH-3 | PH-3 Platform for Series 20 chambers, heater | Login | Login |
| Platform for RC-25F | ||||
| 64-1563 | PM-4 | PM-4 Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
| 64-0287 | PH-4 | PH-4 Platform for Series 20 chambers, heater | Login | Login |
| Replacement Consumables | ||||
| 64-0712 | CS-12R15 | Round cover glass, #1.5 thickness, 12 mm, 100 pack | Login | Login |
| 64-0713 | CS-15R15 | Round cover glass, #1.5 thickness, 15 mm, 100 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| Replacement Parts | ||||
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |