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RC-26 / RC-26G - Open Diamond Bath Imaging Chambers
Product Summary
Large volume open diamond bath recording chamber that can accommodate large specimens such as slice preparations.
- Applications include patch clamp, intracellular/extracellular recording, and imaging
- Can be used with tissue slices or cell cultured coverslips
- Tissue slice anchors available
- Diamond shaped bath provides laminar solution flow
- Large viewing area
- Compatible with upright or inverted microscopy
The RC-26 and RC-26G chambers are designed to be used with cells cultured onto glass coverslips, or with tissue and brain slice samples. The broad imaging area of these chambers (12 x 25 mm and 15 x 24 mm for the RC-26 and RC-26G, respectively) provides excellent access for both electrodes and water-immersion objectives. The diamond shaped geometry facilitates laminar solution flow throughout the bath area.
The large bath of these chambers allows a coverslip to be inserted into the top opening and removed at the end of the experiment. The RC-26 and RC-26G chambers can accept a 12 or 15 mm diameter coverslip, respectively. The working volumes, for 1 mm solution height, of the RC-26 and RC-26G model chambers are 170 and 180 ul, respectively.
Large tissue samples can also be placed directly into the chamber. Slice anchor hold downs (harps) are available for tissue slice studies. Anchors are available with thread spacing (Lycra) at 1.0, 1.5, and 2.0 mm.
Both chambers require a platform (PM-1, PH-1, or P-1) and Series 20 stage adapter to complete the assembly for mounting on a microscope. The chamber floor consists of a 22 x 40 mm coverslip.
Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.
An application video is available for this product through the JoVE website!
You can view our selection of videos and access full length videos here.
| RC-26 | RC-26G | |
|---|---|---|
| Material | Polycarbonate | Polycarbonate |
| Bath dimensions (L x W x H) |
25 x 12 x 4 mm | 24 x 15 x 4 mm |
| Outer dimensions (L x W x H) |
40 x 23 x 4 mm | 40 x 23 x 4 mm |
| Volume, by depth | 150 μl/mm | 180 μl/mm |
| Minimum access angle (from side to center of bath) | 28° | 28° |
| Bottom coverglass | 22 x 40 mm | 22 x 40 mm |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) | 1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order. |
|---|---|---|---|---|
| 64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
References
References
- Tumor necrosis factor-[alpha]-induced neutral sphingomyelinase-2 modulates synaptic plasticity by controlling the membrane insertion of NMDA receptors
Wheeler D, Knapp E, Bandaru VV, Wang Y, Knorr D, Poirier C, Mattson MP, Geiger JD, Haughey NJ
J Neurochem. 2009 Jun;109(5):1237-49. Epub 2009 Mar 14 - Metabolic Acidosis Increases Intracellular Calcium in Bone Cells Through Activation of the Proton Receptor OGR1
Frick KK, Krieger NS, Nehrke K, Bushinsky DA
J Bone Miner Res. 2009 Feb;24(2):305-13 - Protonophore- and pH-insensitive glucose and sucrose accumulation detected by FRET nanosensors in Arabidopsis root tips
Chaudhuri B, Hörmann F, Lalonde S, Brady SM, Orlando DA, Benfey P, Frommer WB
Plant J. 2008 Dec;56(6):948-62. Epub 2008 Sep 18 - Deranged Calcium Signaling and Neurodegeneration in Spinocerebellar Ataxia Type 3
Chen X, Tang TS, Tu H, Nelson O, Pook M, Hammer R, Nukina N, Bezprozvanny I
J Neurosci. 2008 Nov 26;28(48):12713-24 - Evaluation of Dimebon in cellular model of Huntington's disease
Wu J, Li Q, Bezprozvanny I
Mol Neurodegener. 2008 Oct 21;3:15 - Drosophila Painless Is a Ca2+-Requiring Channel Activated by Noxious Heat
Sokabe T, Tsujiuchi S, Kadowaki T, Tominaga M
J Neurosci. 2008 Oct 1;28(40):9929-38 - Massive Ca-induced Membrane Fusion and Phospholipid Changes Triggered by Reverse Na/Ca Exchange in BHK Fibroblasts
Yaradanakul A, Wang TM, Lariccia V, Lin MJ, Shen C, Liu X, Hilgemann DW
J Gen Physiol. 2008 Jul;132(1):29-50. Epub 2008 Jun 18 - Mitochondrial ATP-sensitive K+ channels regulate NMDAR activity in the cortex of the anoxic western painted turtle
Pamenter ME, Shin DS, Cooray M, Buck LT
J Physiol. 2008 Feb 15;586(4):1043-58. Epub 2007 Dec 13 - Pigment Dispersing Factor-Dependent and -Independent Circadian Locomotor Behavioral Rhythms
Sheeba V, Sharma VK, Gu H, Chou YT, O'Dowd DK, Holmes TC
J Neurosci. 2008 Jan 2;28(1):217-27 - AMPA receptors undergo channel arrest in the anoxic turtle cortex
Pamenter ME, Shin DS, Buck LT
Am J Physiol Regul Integr Comp Physiol. 2008 Feb;294(2):R606-13. Epub 2007 Dec 5 - Autocrine activation of EGF receptor promotes oscillation of glutamate-induced calcium increase in astrocytes cultured in rat cerebral cortex,
Mitsuhiro Morita, Nagisa Kozuka, Rurika Itofusa, Masashi Yukawa, Yosihisa Kudo,
Journal of Neurochemistry Volume 95 Issue 3 Page 871-879, November 2005 - Reelin Modulates NMDA Receptor Activity in Cortical Neurons
Ying Chen, Uwe Beffert, Mert Ertunc, Tie-Shan Tang, Ege T. Kavalali, Ilya Bezprozvanny, and Joachim Herz1
The Journal of Neuroscience, September 7, 2005, 25(36):8209-8216 - Src regulates distinct pathways for cell volume control through Vav and phospholipase Cgamma,
Elisabeth T. Barfod, Ann L. Moore, Richard F. Melnick, and Steven D. Lidofsky
J. Biol. Chem., Vol. 280, Issue 27, 25548-25557, July 8, 2005 - Exocytic Trafficking Is Required for Nicotine-induced Up-regulation of 4 2 Nicotinic Acetylcholine Receptors
Tamara Darsow, T. K. Booker, Juan Carlos PinÜ a-Crespo, and Stephen F. Heinemann
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 280, No. 18, Issue of May 6, pp. 18311.18320, 2005 - Ileitis modulates potassium and sodium currents in guinea pig dorsal root ganglia sensory neurons
Stewart T, Beyak MJ, Vanner S
J Physiol. 2003 Nov 1;552(Pt 3):797-807. Epub 2003 Aug 15
Related Products
Related Files
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chambers | ||||
| 64-0234 | RC-26 | Large bath recording chamber, 150 µl volume | Login | Login |
| 64-0235 | RC-26G | Large bath recording chamber, 234 µl volume | Login | Login |
| Platforms | ||||
| 64-1526 | PM-1 | PM-1 Platform for Series 20 chambers, heated, magnetic clamps | Login | Login |
| 64-0284 | PH-1 | PH-1 Platform for Series 20 chambers, heater | Login | Login |
| Slice Anchors (Harps) - RC-26 | ||||
| 64-0265 | SHD-26KIT | Slice anchor kit for RC-26, stainless steel | Login | Login |
| 64-0250 | SHD-26H/10 | Slice anchor for RC-26, 1.0 mm thread spacing, stainless steel | Login | Login |
| 64-0251 | SHD-26H/15 | Slice anchor for RC-26, 1.5 mm thread spacing, stainless steel | Login | Login |
| 64-0252 | SHD-26H/2 | Slice anchor for RC-26, 2.0 mm thread spacing, stainless steel | Login | Login |
| Slice Anchors (Harps) - RC-26G | ||||
| 64-0266 | SHD-26GKIT | Slice anchor kit for RC-26G, stainless steel | Login | Login |
| 64-0253 | SHD-26GH/10 | Slice anchor for RC-26G, 1.0 mm thread spacing, stainless steel | Login | Login |
| 64-0254 | SHD-26GH/15 | Slice anchor for RC-26G, 1.5 mm thread spacing, stainless steel | Login | Login |
| 64-0255 | SHD-26GH/2 | Slice anchor for RC-26G, 2.0 mm thread spacing, stainless steel | Login | Login |
| Replacement Consumables | ||||
| 64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Replacement Parts | ||||
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |