Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-27L) Large Bath Chamber with Slice Supports
Product Summary
A large rectangular bath with a slice support and anchor increases slice viability during long term studies.
- Designed for tissue slice studies
- Permits solution flow both above and below a tissue slice
- Provides good access for immersion optics and electrodes.
- Can be used with either upright or inverted microscopes
- Slice anchors available
The RC-27L is designed with tissue slice studies in mind. The tissue slice rests on a slice support and is held in place by a slice anchor (also known as a harp). This design allows the tissue slice to be perfused from both above and below, which can increase the sample.s viability. The slice support rests 0.5 mm above the coverslip floor and are included with the RC-27L.
Slice anchors are sold separately.
The slice support frame is manufactured in polycarbonate and uses fine Lycra threads to suspend the slice. Thread spacing on the slice support is 1 mm.
Slice anchors are available in polycarbonate or stainless steel, and thread spacing on anchors are 1.0, 1.5, and 2.0 mm which allows for maximum access to the preparation. In addition to individual anchors, Warner offers polycarbonate or stainless steel slice anchor kitscontaining anchors in all available thread spacing.
While the weight of a metal harp is sufficient to secure a slice into place, the plastic harp relies on a close apposition between itself and the chamber side walls to maintain its position. This approach can reduce pressure on the slice from the harp.
The RC-27L requires a platform (PM-6D or PH-6D) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.
Each chamber is supplied with an SS-3 slice support, 10 ft of PE-160 polyethylene tubing, and a package of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, pkg. of 50).
| Material | Polycarbonate |
|---|---|
| Bath dimensions (L x W x H) |
38 x 18 x 3 mm |
| Outer dimensions (L x W x H) |
50 x 25 x 4 - 8 mm |
| Volume, by depth | 560 μl/mm |
| Minimum access angle (from side to center of bath) | 16° |
| Bottom coverglass | 24 x 50 mm |
| Slice support | 24 x 50 mm, Polycarbonate Lycra threads at 1 mm |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order. |
|---|---|---|---|---|
| 64-0709 | CS-24/50 | Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0273 | SS-3 | Standard slice support (1 mm thread spacing) for RC-27L, RC-29 chambers | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
References
References
- Novel mGluR- and CB1R-Independent Suppression of GABA Release Caused by a Contaminant of the Group I Metabotropic Glutamate Receptor Agonist, DHPG
Lafourcade CA, Zhang L, Alger BE
PLoS One. 2009 Jul 1;4(7):e6122 - Downregulation of Tonic GABAergic Inhibition in a Mouse Model of Fragile X Syndrome
Curia G, Papouin T, Séguéla P, Avoli M
Cereb Cortex. 2009 Jul;19(7):1515-20 - Enlarged extracellular space of aquaporin-4–deficient mice does not enhance diffusion of Alexa Fluor 488 or dextran polymers
Xiao F, Hrabetová S
Neuroscience. 2009 Jun 16;161(1):39-45 - Intracellular Zn2+ increases contribute to the progression of excitotoxic Ca2+ increases in apical dendrites of CA1 pyramidal neurons
Vander Jagt TA, Connor JA, Weiss JH, Shuttleworth CW
Neuroscience. 2009 Mar 3;159(1):104-14 - Age-Dependent Alterations of Corticostriatal Activity in the YAC128 Mouse Model of Huntington Disease
Joshi PR, Wu NP, André VM, Cummings DM, Cepeda C, Joyce JA, Carroll JB, Leavitt BR, Hayden MR, Levine MS, Bamford NS
J Neurosci. 2009 Feb 25;29(8):2414-27 - Intracellular Zn2+ Accumulation Contributes to Synaptic Failure, Mitochondrial Depolarization, and Cell Death in an Acute Slice Oxygen–Glucose Deprivation Model of Ischemia
Medvedeva YV, Lin B, Shuttleworth CW, Weiss JH
J Neurosci. 2009 Jan 28;29(4):1105-14 - Diffusion of flexible random-coil dextran polymers measured in anisotropic brain extracellular space by integrative optical imaging
Xiao F, Nicholson C, Hrabe J, Hrabetová S
Biophys J. 2008 Aug;95(3):1382-92 - Vasopressin Increases Locomotion through a V1a Receptor in Orexin/Hypocretin Neurons: Implications for Water Homeostasis
Tsunematsu T, Fu LY, Yamanaka A, Ichiki K, Tanoue A, Sakurai T, van den Pol AN
J Neurosci. 2008 Jan 2;28(1):228-38 - The effects of oleic-acid (OA) on distinct populations of neurons in the hypothalamic arcuate nucleus (ARC) are dependent on extracellular glucose levels
Wang R, Cruciani-Guglielmacci C, Migrenne S, Magnan C, Cotero VE, Routh VH
J Neurophysiol. 2006 Mar;95(3):1491-8 - Influence of Location of a Fluorescent Zinc Probe in Brain Slices on Its Response to Synaptic Activation
Alan R. Kay and Katalin Tóth
J Neurophysiol 95:1949-1956, 2006 - Bupivacaine Inhibits Activation of Neuronal Spinal Extracellular Receptor–activated Kinase through Selective Effects on Ionotropic Receptors
Fumi Yanagidate, M.D., Gary R. Strichartz, Ph.D.
Anesthesiology 2006; 104:805–14 - Cholecystokinin Activates Orexin/Hypocretin Neurons through the Cholecystokinin A Receptor
Natsuko Tsujino, Akihiro Yamanaka, Kanako Ichiki, Yo Muraki, Thomas S. Kilduff, Ken-ichi Yagami, Satoru Takahashi, Katsutoshi Goto, and Takeshi Sakurai1
The Journal of Neuroscience, August 10, 2005, 25(32):7459-7469 - Reactive oxygen species formation in the transition to hypoxia in skeletal muscle
Zuo L, Clanton TL
Am J Physiol Cell Physiol. 2005 Jul;289(1):C207-16 - Input of orexin/hypocretin neurons revealed by a genetically encoded tracer in mice
Sakurai T, Nagata R, Yamanaka A, Kawamura H, Tsujino N, Muraki Y, Kageyama H, Kunita S, Takahashi S, Goto K, Koyama Y, Shioda S, Yanagisawa M
Neuron. 2005 Apr 21;46(2):297-308 - Chronic Cocaine Administration Switches Corticotropin-Releasing Factor2 Receptor-Mediated Depression to Facilitation of Glutamatergic Transmission in the Lateral Septum,
Jie Liu, Baojian Yu, Luis Orozco-Cabal, Dimitri E. Grigoriadis, Jean Rivier, Wylie W. Vale, Patricia Shinnick-Gallagher, and Joel P. Gallagher,
The Journal of Neuroscience, January 19, 2005, 25(3):577-583
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chamber | ||||
| 64-0241 | RC-27L | Large bath chamber for slice studies, does not include slice hold down | Login | Login |
| Platforms | ||||
| 64-1528 | PM-6D | PM-6D Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
| 64-0290 | PH-6D | PH-6D Platform for Series 20 chambers, heater | Login | Login |
| Slice Anchors (Harps) | ||||
| 64-0268 | SHD-27LKIT | Slice anchor kit for RC-27L, RC-27D, RC-29, stainless steel | Login | Login |
| 64-0256 | SHD-27LH/10 | Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, stainless steel | Login | Login |
| 64-0257 | SHD-27LH/15 | Slice anchor for RC-27L, RC-27D, RC-29, 1.5 mm thread spacing, stainless steel | Login | Login |
| 64-0258 | SHD-27LH/2 | Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, stainless steel | Login | Login |
| Slice Anchors (Harps) - Plastic Frame | ||||
| 64-0259 | SHD-27LP/10 | Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, plastic frame | Login | Login |
| 64-0261 | SHD-27LP/2 | Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, plastic frame | Login | Login |
| Replacement Consumables | ||||
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0273 | SS-3 | Standard slice support (1 mm thread spacing) for RC-27L, RC-29 chambers | Login | Login |
| 64-0709 | CS-24/50 | Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| Replacement Parts | ||||
| 64-1450 | CPS-27L | Perfusion spreader plate for RC-27L | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |